Recombinant DNA Technology
Recombinant DNA technology, joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome. Recombinant DNA technology is used extensively in research laboratories worldwide to explore myriad questions about gene structure, function, expression pattern, regulation, and much more. Molecular cloning is the laboratory process used to create recombinant DNA. It is one of two most widely used methods, along with polymerase chain reaction (PCR), used to direct the replication of any specific DNA sequence chosen by the experimentalist. Recombinant DNA techniques are also a cornerstone of the biotechnology industry. This session covers all the aspects in Recombinant DNA Technology.