Mona El Shabrawy,
National Research Centre, Egypt Networking
Biography
Mona El Shabrawy is the head of Microbiology and Immunology department, National Research Centre, Egypt. Her expertise is in conventional and molecular
identification of bacteria especially Staphylococci. She supervised about 15 Master and PhD theses and shared in several projects. Also she is a member of
organizing and scientific committee of three international conferences. Furthermore, she owns and manage EL-Mona laboratory series.
Abstract
Two hundred and twenty six samples were collected from human, dog, cat and poultry and examined for the
existence of coagulase positive staphylococci. The conventional slide method identified 32 staphylococcal isolates
as coagulase positive whereas this number was reduced by tube method to be 20 isolates. Based on Voges-Proskauer
and Polymyxin B resistance tests, the isolates were differentiated into 16 S. aurues and two S. intermedius. The PCR
amplification of thermonuclease and coagulase genes was conducted on S. aurues isolates, and then the coagulase
positive products were put through restriction digestion using RFLP with AluI. Four distinct profiles (I, II, III &IV)
were obtained. The coa gene was sequenced and exhibited a major genotype of S. aureus clone despite the different
species origins supposing a common ancestor. The achieved data put this study in scene when proposed control
strategies of staphylococcal infection as the issues in the regard.